Description

2X premix reagent optimized for dye-based Real-time PCR with enhanced amplification kinetics and superior sensitivity.

• Contains next-generation antibody-mediated Taq DNA Polymerase, ultrapure dNTPs, MgCl₂, and proprietary specificity-enhancing factors
• Requires only primers and template DNA for immediate qPCR setup

• Pre-mixed Universal ROX enables compatibility with Low-ROX, High-ROX, and ROX-independent instruments
• Built-in blue tracking dye allows visual verification of pipetting accuracy and reduces handling errors
• Dual-action enzyme system suppresses primer-dimer formation for highly reproducible results
• Enables linear detection down to single-digit template copies with high sensitivity (R²=0.9994)

RealMOD™ Green T² 2X qPCR mix is a premixed reagent specifically designed for dye-based Real-time PCR applications by maximizing DNA amplification kinetics. This product contains all essential components required for qPCR, including a next-generation antibody-mediated Taq DNA Polymerase, ultrapure dNTPs, MgCl₂, and proprietary specificity-enhancing factors, requiring only the addition of primers and template DNA. The inclusion of a Universal ROX reference dye ensures seamless compatibility across various qPCR platforms without the need for calibration. In addition, a non-interfering blue tracking dye provides real-time visual confirmation during pipetting, reducing human error. The advanced dual-action enzyme system suppresses non-specific amplification and ensures highly reproducible detection.

Applications

• 01Detection and quantification of DNA targets from genomic DNA or cDNA libraries
• 02High-precision gene expression analysis
• 03Quantification of low-copy number templates

Kit Contents

Technical Data

RealMOD™ Green T² 2X qPCR mix is engineered to deliver sharp, high-plateau amplification curves and absolute quantitative accuracy across any DNA concentration, from bulk genomic DNA to low-copy cDNA libraries.

Performance Comparison against Competitor (GAPDH Target)

•​ Assay Profile: Comparative quantification of a standard housekeeping gene target from human genomic DNA template.

•​ Dilution Matrix Parameters: The DNA template was 10-fold serially diluted from 5 ng down to50 pg (executed in duplicate).

•​ Performance Validation: RealMOD™ Green T² 2X qPCR mix (Green curves) demonstrates significantly earlier Ct values, substantially higher final fluorescence intensities (Plateau values), and a highly specific single-target amplification profile compared to Supplier A (Blue curves).

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Linear Dynamic Range for Low-Copy DNA Targets (CPIV cDNA)

•​ Verification Matrix: Validation of a broad linear dynamic range and high-sensitivity quantification infrastructure utilizing a 10-fold serially diluted DNA template (ranging from high concentration down to single-digit copy thresholds) executed in duplicate amplification kinetics.

•​ Experimental Assay Criteria: Dynamic range amplification curves of the targeted DNA region.

•​ Performance Validation: The precisely and evenly spaced amplification curves demonstrate excellent, near-perfect amplification efficiency and highly robust detection capabilities even at ultra-low DNA concentration thresholds. This confirms the kit's broad template compatibility and exceptional performance stability with challenging, low-copy DNA types.