Description

RealMOD™ Probe M² 2X qRT-PCR mix (with UDG) is a 2X concentration premix type reagent specially designed for Real-time RT-PCR by using TaqMan probe.And this kit contains all necessary reagents (DNA Polymerase, UDG, reverse transcriptase, ultrapure dNTPs, dUTP, MgCl₂ etc.) for Real-time RT-PCR reaction except for primers, probe and template RNA. The added UDG-system reagents,dUTP and thermolabile UDG, are included in the mixture to prevent the reamplification of cross/carry over PCR products between reactions. dUTP in the mixure ensures that any amplified cDNA will contain uracil. UDG removes uracil residues from single- or double-stranded cDNA, preventing uracil containing cDNA from serving as template in future PCRs. 

Also, the added anti Taq antibody based on Hot-start DNA polymerase prevents extension of non-specifically annealed primers and primer-dimer formation at low temperatures during qRT-PCR setup. Thus, this RealMOD™ Probe M²​ 2X qRT-PCR mix (with UDG) enables accurate and convenience quantitative analysis over a wide range of template RNA concentrations. A ready-to-use solution is optimized for Real-time qRT-PCR analysis.

●​ Minimize cross contamination through UDG application

●​ Real time RT PCR reaction volume can be controlled with 2X Master mix type

●​ Hot start function included to minimize non-specific reactions

●​ Excellent reproducibility and stability

●​ Confirmation of excellent qRT PCR results even in templates with high GC contents​