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[LiliF] Total Avian Diagnostics Solution
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LiliF_AIV_Total_Solution.pdf

[Brochure]_LiliF AIV Total solution.pdf

Date 01-Sep-26 Hit 562
iNtRON Biotechnology
Diagnostics Division

LiliF® Avian MDx

TOTAL AVIAN DIAGNOSTICS SOLUTION

Complete molecular workflow from screening to confirmation

Avian Influenza Virus (AIV)

Avian influenza (AI), caused by influenza A virus, affects a wide range of birds, from chickens and turkeys to wild and pet birds. Some strains, particularly the highly pathogenic H5N1, can lead to high mortality and have drawn global attention due to outbreaks in wild birds. While generally species-specific, H5N1 can also infect mammals, including humans, making it a virus of significant concern.

Source: OIE Terrestrial Animal Health
✓ Full Line-up System in Avian Disease Diagnosis
Provides a complete molecular diagnostic solution from screening to confirmation using conventional RT-PCR and Real-time PCR assays.
✓ Reliable and Confident Results
Incorporates internal positive controls and advanced primer technologies to provide highly accurate and consistent detection of avian pathogens.
✓ Fast, Accurate, and User-Friendly
Streamlines the veterinary testing workflow with Maxime™ dried PCR premixes, ensuring excellent sensitivity, specificity, and repeatability.
WORK FLOW

Diagnostic Products & Mechanisms

Each LiliF® kit is engineered with dedicated molecular principles optimized for screening, subtyping, and quantitation.

A. One-Step Conventional RT-PCR Subtyping Systems

Endpoint RT-PCR
Target: Qualitative subtyping of all 16 Hemagglutinin (HA) serotypes (H1–H16) of Avian Influenza Virus (AIV).
Diagnostic Principle:

Employs one-step Reverse Transcription PCR (RT-PCR) to amplify extracted viral RNA in a single tube. Incorporates CLP™ (Customized Linker Primer) technology, which controls non-specific priming and optimizes Tm conditions to maximize specificity and sensitivity. Provided in a Maxime™ dried premix format to enhance pipetting precision, and contains 8-MOP to prevent post-PCR carry-over contamination. Results are visualized by agarose gel electrophoresis.

LiliF® AIV Multi-tube RT-PCR Kit

Cat. IPC11009 | Size: 12 T
Target: Qualitative screening of Avian Influenza Virus (AIV), targeting NP, M, H5, H7, and H9 genes.
Diagnostic Principle:

Utilizes one-step RT-PCR combining reverse transcription of viral genomic RNA into cDNA with subsequent PCR amplification in a single reaction. Employs CLP™ technology to prevent non-specific band formation and Maxime™ dry-lyophilized master mix pre-dispensed in strip tubes. This eliminates manual master mix aliquoting steps, minimizing contamination risk and error. Target bands are verified via standard gel electrophoresis.

B. Probe-based One-Step Real-time RT-PCR Detection Systems

qRT-PCR Detection

LiliF® AIV M Real-time RT-PCR Kit

Cat. IP15004 | Size: 48 T
Target: Qualitative screening of Type A Influenza Virus by amplifying the highly conserved M (Matrix) gene.
Diagnostic Principle:

Employs probe-based Real-time Reverse Transcription PCR. RNA extracted from specimens is converted into cDNA and amplified using primers specific to the highly conserved M gene. Fluorescent TaqMan probes target this sequence in real-time, emitting fluorescence detected by the qPCR optical module during the cycle. Incorporates high-performance RTase and Hot Start DNA polymerase in a stabilized premix to maximize sensitivity and avoid false positives.

LiliF® AIV H5 Real-time RT-PCR Kit

Cat. IP15005 | Size: 48 T
Target: Specific typing of H5 subtype (Highly Pathogenic Avian Influenza) targeting the H5 (HA) gene.
Diagnostic Principle:

Combines reverse transcription and real-time PCR amplification in a single tube using TaqMan probe-based chemistry. Focuses on the H5-specific sequence of the Hemagglutinin gene. The fluorescent probe, upon binding to the amplified target cDNA, is cleaved by the 5' nuclease activity of the DNA polymerase, generating real-time fluorescence signals. Includes internal positive controls (IPC) to verify reaction efficiency and safeguard clinical quarantine diagnostics.

LiliF® AIV H7 Real-time RT-PCR Kit

Cat. IP15006 | Size: 48 T
Target: Specific typing of H7 subtype Avian Influenza Virus by targeting the H7 (HA) gene.
Diagnostic Principle:

Developed in coordination with the Animal and Plant Quarantine Agency (APQA) for standardized genetic testing. Operates on real-time probe-based RT-PCR. Targeting the specific H7 hemagglutinin sequence, it monitors cDNA synthesis and exponential DNA amplification using sequence-specific primers and fluorophore-quenched probes, ensuring unmatched typing precision and high analytical sensitivity.

LiliF® IBV Real-time RT-PCR Kit

Cat. IP15281 | Size: 50 T
Target: Specific detection of Infectious Bronchitis Virus (IBV), a highly contagious poultry coronavirus.
Diagnostic Principle:

Utilizes probe-based real-time PCR targeting the highly conserved regions of the IBV viral genome. The one-step RT-PCR reaction combines reverse transcriptase and Hot Start PCR polymerase to convert single-stranded RNA into cDNA and amplify it. Incorporates CLP™ technology to prevent primer-dimer formation and non-specific priming. A dual-channel optical profile detects the target pathogen and an Internal Positive Control (IPC) to prevent false-negative interpretation.

LiliF® NDV Real-time RT-PCR Kit

Cat. IP15456 | Size: 50 T
Target: Specific detection of Newcastle Disease Virus (NDV), a devastating avian paramyxovirus.
Diagnostic Principle:

Designed as a probe-based one-step real-time RT-PCR. Targets NDV genomic RNA, performing cDNA synthesis and amplification simultaneously in a stabilized master mix. Utilizes 5' nuclease TaqMan probe chemistry to isolate the NDV signal in the primary channel while monitoring amplification validation in the HEX/VIC channel via a pre-mixed internal control (IPC), ensuring robust diagnostics in diagnostic labs.

Avian Diagnostics Ordering Information

Catalog
Cat. No. Product Name Technology Volume
IPC12020 LiliF® AIV HA (1-16) Multi-tube RT-PCR Kit Conventional RT-PCR 6 Tests
IPC11009 LiliF® AIV Multi-tube RT-PCR Kit Conventional RT-PCR 12 Tests
IP15004 LiliF® AIV M Real-time RT-PCR Kit Real-time RT-PCR 48 Tests
IP15005 LiliF® AIV H5 Real-time RT-PCR Kit Real-time RT-PCR 48 Tests
IP15006 LiliF® AIV H7 Real-time RT-PCR Kit Real-time RT-PCR 48 Tests
IP15281 LiliF® IBV Real-time RT-PCR Kit Real-time RT-PCR 50 Tests
IP15456 LiliF® NDV Real-time RT-PCR Kit Real-time RT-PCR 50 Tests

List

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