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Human MDx

LiliF Diagnostic Kits MDx Human MDx

G-spin™ Total DNA Extraction Mini Kit

Cat.No	Capacity	Inquire
17045	50 col.
17046	200 col.

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Description
TroubleShooting Guide
Related products
Protocol
MSDS

PRODUCT INFORMATION

Description

Spin type product for extracting high yield and high purified DNA from various specimen such as Whole Blood, Cell, Tissue, Fixed tissue and Gram (-) Bacteria.

• Possible to extract DNA from various specimens such as Whole Blood, Cell, Tissue, Fixed tissue and Gram (-) Bacteria
• Possible to extract high yield and purified DNA with upgraded Lysis Buffer and Binding Buffer
• In case for extracting specimen from Blood, DNA can be extracted with only one buffer without performing RBC lysis separately.
• Genomic DNA extraction can be done quickly within 20~30 minutes.
• Optimized protocol for each specimen is provided.

G-spin™ Total DNA Extraction Mini Kit is instructed to extract genomic DNA simply and quickly from animal culture cell, blood, animal tissue, Rodent tail, fixed tissue, hair and Gram negative bacteria. This product provides upgraded Lysis Buffer and Binding Buffer compare to conventional G-spin™ Series in order to extract high yield and purified DNA from various specimens but also make possible to extract from blood without any process needed for RBC lysis. Also, it removes inhibitor in order to be used in downstream applications such as PCR and hybridization research. Also, it provides optimized protocol for each specimens (Table 1. Reference), therefore there is very high reproducibility for test results and expedite experiments. G-spin™ Total DNA Extraction Mini Kit verified its performance through testing various types of specimens.

Table 1. Protocols of G-spinTM Total DNA Extraction Mini Kit

Applications

01Cancer research
02Gram negative bacterial research
03Human genetic research
04Viral DNA Research
05Detection Assay : PCR, real time PCR
06DNA hybridization : Southern blotting, microarray

Kit Contents

Contents	50 COLUMNS	200 COLUMNS
Buffer CL	25ml × 1 ea	90ml × 1 ea
Buffer BL	25ml × 1 ea	90ml × 1 ea
Buffer WA	40ml × 1 ea	160ml × 1 ea
Buffer WB (Concentration)	10ml / 5ml	40ml / 20ml
Buffer CE	20ml × 1 ea	40ml × 1 ea
Spin Column (Green color) & Collection tube	50 ea	200 ea
RNase A, lyophilized	3 mg x 1 vial	3 mg x 4 vial
Proteinase K, lyophilized	22 mg x 1 vial	22 mg x 4 vial
Manual	1 ea	1 ea

Technical Data

Recovery rates based on various specimens

After extracting DNA from various types of samples using G-spinTM Total Genomic DNA Extraction kit, high yield and purified DNA is identified from animal cell, blood, tissue, gram-negative bacteria and biological swab.

After loading 100ng of extracted DNA using G-spinTM Total Genomic DNA Extraction Mini Kit on 0.8 % agarose gel(including 1X RedSafe), intact genomic DNA is identified.

Lane M, DNA Marker, lane 1, K562 cell; lane 2, SNU-1 cell; lane 3, U937 cell; lane 4, HeLa cell; lane 5, NIH/3T3 cell; lane 6, Vero cell; lane 7, B16 cell; lane 8, Liver(mouse); lane 9, Heart (mouse); lane 10, Lung(mouse); lane 11, Brain(mouse); lane 12, Kidney(mouse); lane 13, Spleen(mouse); lane 14, muscle(mouse); lane 15, stomach(mouse); lane 16, Tail(mouse); lane 17, Hair(human); lane 18, blood(treated EDTA); lane 19, blood(treated Heparin); lane 20, blood(treated citrate); lane 21, Buffy coat 1; lane 22, Buffy coat 2; lane 23, E. coli; lane 24, P. aeruginosa; lane 25, S. gallinarium; lane 26, Blood swab; lane 27, Buccal swab

DNA extraction Test regarding to Blood sample

In order to observe efficiency of genomic DNA extraction from blood, comparison data between competitors was obtained. Purity of extracted DNA was good when DNA was extracted from 6.25 μl to 200ul of blood volume. Efficiency of DNA extraction was excellent.

After observing DNA’s recovery rate by repeating DNA extraction third time from blood samples obtained by five different contributors, although there is discrepancy between blood contributors, stable recovery of DNA with high reproducibility was identified.

Citation List

1	Ann Surg Treat Res. 2014 Mar;86(3):136-142. English. Published online February 24, 2014. https://doi.org/10.4174/astr.2014.86.3.136	Circulating cell-free DNA as a promising biomarker in patients with gastric cancer: diagnostic validity and significant reduction of cfDNA after surgical resection	Kyongchol Kim, Dong Gue Shin,1 Min Koo Park,2 Seung Hyuk Baik,3 Tae Hee Kim,4 Sanghee Kim,5 and SaeYoung Lee6
2	Oncotarget. 2015 Aug 28; 6(25): 20875–20884. Published online 2015 Aug 17. doi: 10.18632/oncotarget.5205	PRDX6 controls multiple sclerosis by suppressing inflammation and blood brain barrier disruption	Hyung-Mun Yun,1 Kyung-Ran Park,1 Eun-Cheol Kim,1 and Jin Tae Hong2
3	Scientific Reports volume 5, Article number: 15155 (2015) doi:10.1038/srep15155	Complete mitochondrial genome of Bactrocera arecae (Insecta: Tephritidae) by next-generation sequencing and molecular phylogeny of Dacini tribe	Hoi-Sen Yong, Sze-Looi Song, Phaik-Eem Lim, Kok-Gan Chan, Wan-Loo Chow & Praphathip Eamsobhana
4	Published: April 10, 2014 https://doi.org/10.1371/journal.pone.0088453	Transovarial Transmission of Orientia tsutsugamushi in Leptotrombidium palpale (Acari: Trombiculidae)	Eun Hee Shin, Jong Yul Roh, Won Il Park, Bong Gu Song, Kyu-Sik Chang, Wook-Gyo Lee, Hee Il Lee, Chan Park, Mi-Yeoun Park,E-Hyun Shin
5	Korean J Anesthesiol. 2014 Apr;66(4):295-299. English. Published online April 28, 2014. https://doi.org/10.4097/kjae.2014.66.4.295	Multidrug-resistant Acinetobacter baumannii infection in intensive care unit patients in a hospital with building construction: is there an association?	Sepideh Kamalbeik,1 Haleh Talaie,2 Arezou Mahdavinejad,1 Abdollah Karimi,3 and Alireza Salimi
6	doi: 10.1128/genomeA.01298-14 Genome Announc. November/December 2014 vol. 2 no. 6 e01298-14	Whole-Genome Sequence of Borrelia garinii Strain 935T Isolated from Ixodes persulcatus in South Korea	Yoontae Noha, Su Yeon Kima, Yeong Seon Leea, Dae-Won Kimb, Taesoo Kwonb, Kyu-Jam Hwanga
7	Asian-Australas J Anim Sci. 2017 Aug; 30(8): 1099–1104. Published online 2017 Jan 26	Effect of alcohol dehydrogenase 1C (ADH1C) genotype on vitamin A restriction and marbling in Korean native steers	Dong Qiao Peng,1,2 U Suk Jung,1 Jae Sung Lee,1,2 Won Seob Kim,1,2 Yong Ho Jo,1,2 Min Jeong Kim,1 Young Kun Oh,3 Youl Chang Baek,3 Seong Gu Hwang,4 and Hong Gu Lee1
8	Volume 64, Issue 1 February 2017 Pages 167–170	First Experimental Evidence for the Transmission of Chlamydia psittaci in Poultry through Eggshell Penetration	Ahmed, De Boeck, Dumont,Cox, K. De Reu,D. Vanrompay
9	February 14, 2018 https://doi.org/10.1371/journal.pone.0192637	Diagnosis of amphimeriasis by LAMPhimerus assay in human stool samples long-term storage onto filter paper	William Cevallos , Pedro Fernández-Soto , Manuel Calvopiña, María Buendía-Sánchez, Julio López-Abán, Belén Vicente, Antonio Muro
10	Current Microbiology September 2017, Volume 74, Issue 9, pp 1076–1082	High Diversity of Bacterial Communities in Developmental Stages of Bactrocera carambolae (Insecta: Tephritidae) Revealed by Illumina MiSeq Sequencing of 16S rRNA Gene	Hoi-Sen Yong, Sze-Looi Song, Kah-Ooi Chua, Phaik-Eem Lim
11	Meta Gene Volume 14, December 2017, Pages 6-11	Predominance of Wolbachia endosymbiont in the microbiota across life stages of Bactrocera latifrons (Insecta: Tephritidae)	Hoi-Sen Yong, Sze-Looi Song , Kah-Ooi Chua, Phaik-Eem Lim
12	Journal of Asia-Pacific Entomology Volume 20, Issue 3, September 2017, Pages 809-813	Host-feeding pattern and dengue virus detection of Aedes albopictus (Diptera: Culicidae) captured in an urban park in Korea	Hyunwoo Kim a, b, Hye mi Yu a, Hyung Woo Lim a, Sung-Chan Yang a, Jong Yul Roh a, Kyu Sik Chang a, E-Hyun Shin a, Young Ran Ju a, Wook-Gyo Lee
13	Vet World. 2017 Jun; 10(6): 610–615. Published online 2017 Jun 8.	Using real-time polymerase chain reaction as an alternative rapid method for enumeration of colony count in live Brucella vaccines	Waleed S. Shell, Mahmoud L. Sayed, A. A. Samy, Ghada Mohamed Al-Sadek,1 Gina Mohamed Mohamed Abd El-Hamid, Abdel Hakam M. Ali
14	Volume 144, Issue 13 November 2017 , pp. 1821-1827	First detection of Echinococcus multilocularis in rodent intermediate hosts in Turkey	HAMZA AVCIOGLU , ESIN GUVEN, IBRAHIM BALKAYA , RIDVAN KIRMAN
15	January 25, 2017 https://doi.org/10.3892/ol.2017.5647 Pages:1811-1818	Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors	Ann Kakabadze, Konstantine Mardaleishvili, George Loladze,Lia Karalashvili, Gocha Chutkerashvili,David Chakhunashvili, Zurab Kakabadze
16	Insect Biochemistry and Molecular Biology Volume 89, October 2017, Pages 31-42	TmSR-C, scavenger receptor class C, plays a pivotal role in antifungal and antibacterial immunity in the coleopteran insect Tenebrio molitor	Soo Gon Kim , Yong Hun Jo, Jeong Hwan Seong , Ki Beom Park , Mi Young Noh, Jun Ho Cho, Hye Jin Ko, Chang Eun Kim , Hamisi Tindwa, Bharat Bhusan Patnaik , In Seok Bang , Yong Seok Lee, Yeon Soo Han
17	Jundishapur Journal of Microbiology: March 2017, 10 (3); e40855.Published Online: February 6, 2017	The Prevalence and Genotype Distribution of Human Papillomavirus Types in the General Female Population in West of Iran	Somayeh Jalilian , Babak Izadi ,Seyed Hamid Madani , Parviz Mohajeri
18	Published online: 07 February 2018	Echinococcus multilocularis in a Eurasian lynx (Lynx lynx) in Turkey	Hamza Avcioglu (a1), Esin Guven (a1), Ibrahim Balkaya (a1) and Ridvan Kirman
19	Gut Pathogens20179:32 https://doi.org/10.1186/s13099-017-0181-1 The Author(s) 2017, Received: 13 March 2017, Accepted: 25 May 2017, Published: 2 June 2017	Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea	Yeon Bee Kim, Joon Yong Kim, Hye Seon Song, Changsu Lee, Joseph Kwon, Jisu Kang, Jin-Kyu Rhee, Myeong Seon Jeong, Young-Do Nam† and Seong Woon Roh
20	Meta Gene Volume 11, February 2017, Pages 189-196	Microbiota associated with Bactrocera carambolae and B. dorsalis (Insecta: Tephritidae) revealed by next-generation sequencing of 16S rRNA gene	Hoi-Sen Yong a, b, Sze-Looi Song c, Kah-Ooi Chua a, b, Phaik-Eem Lim c
21	Accepted date: June 03, 2017; Published date: June 09, 2017	Epigenetics Reprogramming of Autophagy is involved in Childhood Acute Lymphatic Leukemi	Dahb M Hassen1, Khalid Bassiouny1, Farha El-Shenawy2 and Hany Khalil1*
22	African Journal of Clinical and Experimental Microbiology,Vol 18, No 1 (2017)	Epidemiologic characteristics of Klebsiella pneumoniae isolates in ventilator-associated pneumonia in intensive care units	A.A. El Sharkawy, M.A. Mansour, H.M. Helmy, D.M. Abd El Azeem
23	Arch Clin Infect Dis. 2017 ;12(1):e39666	Detection of Human Papilloma Virus Type 16 in Epithelial Ovarian Tumors Samples	Masoud Dadashi,Gita Eslam , Ebrahim Faghihloo, Ali Pourmohammad , Jalil Hosseini , Robabeh Taheripanah and Zahra Arab-Mazar
24	Volume 43, Issue 5 May 2017 Pages 923–928	CD40 polymorphism in cervical carcinoma in a subset of Malaysian population	Purushotham Krishnappa, Hong Mun Kong,Ibtisam Binti Mohamad,Kenny Voon,Sushela Devi Somanath
25	31 August 2017, Journal of Medical Microbiology 66: 1324-1327	Molecular evidence for the absence of an association between Simkania negevensis and respiratory diseases	Hesham M. Al-Younes1, Wael Al-Zereini2, Nathir M. Obeidat3

TroubleShooting Guide

QRNase A and Proteinase K contained in the product are in the form of powder. What should be used to dissolve the buffer? What is the concentration after melting?: ARNase A and Proteinase K of this product are supplied as "Lyophilized" and dissolved in pure DW. In case of RNase A, it is supplied in 3mg and dissolved in 0.3ml pure DW, the concentration is 10mg / ml. Proteinase K is supplied in 22 mg and is dissolved in 1.1 ml pure DW at a concentration of 20 mg / ml. Experiment with the amount given in the protocol according to the sample. -20 ℃). After receiving the product, spin down the RNase A and Proteinase K tubes before dissolving and then put in pure DW.

QIt is called total DNA. Does it mean whole genome extraction kit?: AThis product is "Total" which means that gDNA can be extracted from most samples such as whole blood, cell, tissue, fixed tissue and Gram (-) bacteria. gDNA extraction from the sample is possible.

QWe use the Blood gDNA extraction kit. I used G-spin Blood for the first time and now I am using i-genomic Blood product. i-genomic Blood was easy to process with RBC lysis and cell lysis step at once. How does whole blood lysis of G-spin Total product proceed?: AThank you for using our product. The G-spin Total DNA Extraction Mini Kit consists of "RBC and Cell lysis at once," which users felt convenient. In addition, up-grade of lysis buffer and binding buffer improves inhibitor removal efficiency.

QWe want to extract gDNA from paraffin block tissue. Is there an extractable product?: AExtraction is possible with G-spin Total DNA Extraction mini kit. It is possible to extract gDNA from paraffin embedded block and formalin fixed tissue. You can check the pre-treatment and extraction method in "16 page - Protocol E" of the supplied product manual.