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Life Science (Reagent) Extraction Reagent RNA

Viral Gene-spin™ Viral DNA/RNA Extraction Kit

Cat.No	Capacity	Inquire
17151	50 col.

Inquire List

Description
TroubleShooting Guide
Related products
Protocol
MSDS

PRODUCT INFORMATION

Description

Spin type product that can extract viral DNA/RNA easily and rapidly from the various clinical samples such as serum, plasma, cell free body fluid and stool etc.

• Extraction of Viral DNA/RNA from various clinical samples within 20 minutes
• High yield and purity viral DNA / RNA extraction with powerful lysis buffer
• Easy extraction without enzyme treatment
• Be used safely with non-phenol method and no need for EtOH precipitation
• OIE listed product based on various clinical sample results
• Be useful for molecular diagnostic studies on clinical samples by minimizing contamination by CAPS

Viral Gene-spin™ Viral DNA/RNA Extraction Kit is designed to extract virus DNA/RNA easily and quickly from the various clinical samples such as serum, plasma, cell-free fluid, stool, whole blood, tissue, swab, urine, cultured cell and virus infection tissue, etc. Viral Gene-spin™ Viral DNA/RNA Extraction Kit has a very powerful lysis buffer that effectively dissolves and denatures virus envelope and capside protein without additional enzyme treatment (except some viruses) to easily elute viral gene and effectively inactivate RNase. The binding buffer added after the elution helps to attach genes only to silica-gel membrane, and two different washing buffers efficiently remove proteins and other contaminants to get high purity viral gene.

The Viral Gene-spin™ Viral DNA/RNA Extraction Kit does not use harmful organic solvents, so it can be used safely. Even it can extract pure viral gene very quickly without EtOH precipitation. In addition, CAPS is applied to columns to minimize the risk of contamination (see Figure 1). The viral genes isolated and purified from the Viral Gene-spin™ Kit can be used for viral diagnostics, cancer research etc. Especially, it is useful for clinical diagnosis of people or animals that have been infected by virus or bacteria.

Figure 1. Spin Column & CAPS Information

Applications

01Pathogen detection
02Microarray
03cDNA synthesis
04Reverse Transcriptase PCR(RT-PCR)
05Quantitative PCR(qPCR, qRT-PCR) etc.

Kit Contents

Contents	50 COLUMNS
Lysis Buffer	30ml × 1 ea
Binding Buffer	40ml × 1 ea
Washing Buffer A	30ml × 1 ea
Washing Buffer B(Concentration)	10ml × 1 ea
Elution Buffer	20ml × 1 ea
Spin Columns(Orange color)	50 Columns
Collection Tubes	50 tubes
Manual	1 ea

Technical Data

Sensitivity test of HBV (Human hepatitis B virus, DNA virus)

HBV DNA was extracted from sera of HBV-infected patients using the Viral Gene-spin™ Viral DNA/RNA Extraction Kit. As a result of sensitivity test, PCR inhibitions were less because cross-contaminants were completely removed.

Detection limit test of Enterovirus (RNA virus) by Real-time RT-PCR

Viral Gene-spin™ Viral DNA/RNA Extraction Kit was used to extract EV RNA from stool suspension of EV-infected patients, then Real-time RT-PCR was performed. As the result, we could see that viral RNA was properly isolated by the Viral Gene-spin™ Viral DNA/RNA Extraction Kit.

Citation List

1	Tropical Animal Health and Production January 2014, Volume 46, Issue 1, pp 271–277	Molecular epidemiology of Newcastle disease viruses in Vietnam	Kang-Seuk Choi Email author , Soo-Jeong Kye, Ji-Ye Kim, Thanh Long To, Dang Tho Nguyen, Youn-Jeong Lee, Jun-Gu Choi, Hyun-Mi Kang, Kwang-Il Kim, Byung-Min Song, Hee-Soo Lee
2	2014 Summer; 5(3): 187–191.	Phylogenetic characterization of the fusion genes of the Newcastle disease viruses isolated in Fars province poultry farms during 2009-2011	Mohammad Javad Mehrabanpour,1,* Setareh Khoobyar,2 Abdollah Rahimian,1 Mohammad Bagher Nazari,3 and Mohammad Reza Keshtkar4
3	November 2014, Volume 98, Number 11 Page 1590	First Report of Chickpea chlorotic dwarf virus Infecting Hot Pepper in India	H.-S. Byun, E.-J. Kil, S. Kim, and H. Hwang, Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea; J. H. Lee, Nongwoo Bio, Suwon, Korea; Y.-J. Chung,
4	8 January 2014, doi: 10.1128/CVI.00636-13 Clin Vaccine Immunol March 2014 vol. 21 no. 3 360-365	Virus-Like Particle Vaccine Confers Protection against a Lethal Newcastle Disease Virus Challenge in Chickens and Allows a Strategy of Differentiating Infected from Vaccinated Animals	Jae-Keun Parka, Dong-Hun Leea, Seong-Su Yuka, Erdene-Ochir Tseren-Ochira, Jung-Hoon Kwona, Jin-Yong Noha, Byoung-Yoon Kima, Soo-Won Choia, Sang-Moo Kangb, Joong-Bok Leea, Seung-Yong Parka,In-Soo Choia and Chang-Seon Songa
5	Journal Avian Pathology  Volume 44, 2015 - Issue 1	Genetic characterization of three novel chicken parvovirus strains based on analysis of their coding sequences	Bon-Sang Koo, Hae-Rim Lee, Eun-Ok Jeon, Moo-Sung Han, Kyeong-Cheol Min, Seung-Baek Lee
6	J Bacteriol Virol. 2015 Dec;45(4):319-327. English.	Biological Property of Recombinant Hemagglutinin-Neuraminidase Protein of Avian Paramyxovirus Type 6 Expressed by Recombinant Baculovirus	Ji-Ye Kim,1 Hyun-Jeong Lee,1 Soo-Jeong Kye,2 Saeromi Kim,1 Hee-Jung Seul,1 Sang-Eun Kim,4 Hee-Soo Lee,3 Suk-Chan Jung,1 and Kang-Seuk Choi
7	Korean J Intern Med. 2015 Jan; 30(1): 96–103.	Utilization of the respiratory virus multiplex reverse transcription-polymerase chain reaction test for adult patients at a Korean tertiary care center	Mi Young Ahn,1 Seong-Ho Choi,corresponding author1 Jin-Won Chung,1 and Hye Ryoun Kim2
8	Plant Pathology, Volume64, Issue6 December 2015 Pages 1284-1291	Seed transmission of Sweet potato leaf curl virus in sweet potato (Ipomoea batatas)	J. Kim , E.‐J. Kil , S. Kim , H. Seo, H.‐S. Byun , J. Park, M.‐N. Chung , H.‐R. Kwak,
9	J. Microbiol. Biotechnol. (2016), 26(12), 2206–2213 http://dx.doi.org/10.4014/jmb.1609.09012	Identification of Uncommon Candida Species Using Commercial Identification Systems	Tae-Hyoung Kim1, Oh Joo Kweon2, Hye Ryoun Kim2, and Mi-Kyung Lee
10	Future Virology, Vol. 11, No. 3 \| , Published Online:22 Feb 2016https://doi.org/10.2217/fvl-2015-0017	Human metapneumovirus frequency in Iranian children with respiratory symptoms	A Sanaei Dashti, SMH Emamifar, T Hashempour
11	http://dx.doi.org/10.1136/bmjopen-2016-012330	Cluster randomised controlled trial to examine medical mask use as source control for people with respiratory illness	Chandini Raina MacIntyre1,2, Yi Zhang3, Abrar Ahmad Chughtai1,2, Holly Seale1,2, Daitao Zhang3, Yanhui Chu3, Haiyan Zhang3, Bayzidur Rahman1,2, Quanyi Wang3
12	Scientific Reports volume 6, Article number: 19013 (2016) doi:10.1038/srep19013	Tomato yellow leaf curl virus (TYLCV-IL): a seed-transmissible geminivirus in tomatoes	Eui-Joon Kil, Sunhoo Kim, Ye-Ji Lee, Hee-Seong Byun, Jungho Park, Haneul Seo, Chang-Seok Kim, Jae-Kyoung Shim, Jung-Hwan Lee, Ji-Kwang Kim, Kyeong-Yeoll Lee, Hong-Soo Choi & Sukchan Lee
13	19 Jan 2016https://doi.org/10.1089/vbz.2015.1832	First Isolation of Severe Fever with Thrombocytopenia Syndrome Virus from Haemaphysalis longicornis Ticks Collected in Severe Fever with Thrombocytopenia Syndrome Outbreak Areas in the Republic of Korea	Roh Jong Yul , Lee Ye-Ji , Park Won Il , Han Myung Guk , Ju Young Ran and Lee Won-Ja
14	Vector-Borne and Zoonotic DiseasesVol. 16, No. 1	First Isolation of Severe Fever with Thrombocytopenia Syndrome Virus from Haemaphysalis longicornis Ticks Collected in Severe Fever with Thrombocytopenia Syndrome Outbreak Areas in the Republic of Korea	Yun Seok-Min , Song Bong Gu , Choi WooYoung , Roh Jong Yul , Lee Ye-Ji , Park Won Il , Han Myung Guk , Ju Young Ran , and Lee Won-Ja
15	September 2016, Volume 100, Number 9 Page 1958 https://doi.org/10.1094/PDIS-04-16-0424-PDN	First Report of Papaya leaf curl virus in Papayas in Korea and Recovery of its Symptoms	H.-S. Byun, E.-J. Kil, and H. Seo,J.-K. Kim
16	J Vet Sci. 2016 Sep;17(3):323-330. English. Published online September 20, 2016. https://doi.org/10.4142/jvs.2016.17.3.323	The current epidemiological status of infectious coryza and efficacy of PoulShot Coryza in specific pathogen-free chickens	Moo-Sung Han,1 Jong-Nyeo Kim,1 Eun-Ok Jeon,1 Hae-Rim Lee,1 Bon-Sang Koo,1 Kyeong-Cheol Min,1 Seung-Baek Lee,1 Yeon-Ji Bae,1 Jong-Suk Mo,1 Sun-Hyung Cho,1 Hye-Sun Jang,2 and In-Pil Mo
17	J Vet Sci. 2017 Mar;18(1):89-94. English.	Phylogeographical characterization of H5N8 viruses isolated from poultry and wild birds during 2014–2016 in South Korea	Byung-Min Song, Eun-Kyoung Lee, Yu-Na Lee, Gyeong-Beom Heo, Hee-Soo Lee and Youn-Jeong Lee
18	Journal of Wildlife Diseases 53(4):749-760. 2017	MOLECULAR EPIDEMIOLOGY OF AVIAN POXVIRUS IN THE ORIENTAL TURTLE DOVE (STREPTOPELIA ORIENTALIS) AND THE BITING MIDGE (CULICOIDES ARAKAWAE) IN THE REPUBLIC OF KOREA	Hae Rim Lee, Bon-Sang Koo, Jong-Taek Kim, Heung-Chul Kim, Myung-Soon Kim, Terry A. Klein, Man-Seok Shin, Sanghun Lee, Eun-Ok Jeon, Kyung-Cheol Min, Seung Baek Lee, Yeonji Bae
19	Veterinary Microbiology Volume 207, August 2017, Pages 178-180	Prevalence of novel porcine circovirus 3 in Korean pig populations	Taeyong Kwon a, Sung J. Yoo a, Choi-Kyu Park b, Young S. Lyoo a
20	Veterinary Microbiology Volume 199, February 2017, Pages 54-61	Genetic diversity of ORF 4–6 of type 1 porcine reproductive and respiratory syndrome virus in naturally infected pigs	Dong-Uk Lee a, Sung J. Yoo a, Taeyong Kwon a, Sang H. Je a, Jeong Y. Shin a, Jeong J. Byun a, Myung H. Kim b, Young S. Lyoo a
21	Molecular Pathology in Clinical Practice pp 755-778	Respiratory Infections	Christine C. Ginocchio
22	Aquaculture Reports Volume 7, August 2017, Pages 57-65 open access	The presence of Vibrionaceae, Betanodavirus and Iridovirus in marine cage-cultured fish: Role of fish size, water physicochemical parameters and relationships among the pathogens	AzilaAbdullahaRimatulhanaRamliaMohd Syafiq MohammadRidzuanaMuniraMurniaShahidanHashimaFahmiSudirwanaSiti ZahrahAbdullahaNur NazifahMansorbSofeaAmirabMohd ZamriSaadceMohammad Noor AzmaiAmal
23	Vol 7, No 2 (2018) > Kaleibar	Prevalence of Bovine Immunodeficiency Virus Infection in Buffaloes in East Azerbaijan, Northwestern Iran	Mohammad Tolouei- Kaleibar, Morteza Mozaffari, Javad Ashrafi, Golamreza Nikbakht, Ezzatollah Fathi
24	Viruses 2018, 10(2), 78; doi:10.3390/v10020078	First Diagnosed Case of Camelpox Virus in Israel	Oran Erster 1,†, Sharon Melamed 2,†, Nir Paran 2, Shay Weiss 2, Yevgeny Khinich 1, Boris Gelman 1, Aharon Solomony 3 and Orly Laskar-Levy
25	Received: 28 March 2018 \| Revised: 29 May 2018 \| Accepted: 31 May 2018 DOI: 10.1111/jfd.12843	] First detection of tilapia lake virus (TiLV) in wild river carp (Barbonymus schwanenfeldii) at Timah Tasoh Lake, Malaysia	Azila Abdullah1 \| Rimatulhana Ramly1 \| Mohammad Syafiq Mohammad Ridzwan1 \| Fahmi Sudirwan1 \| Adnan Abas2 \| Kamisa Ahmad1 \| Munira Murni1 \| Beng Chu Kua1
26	Journal of Clinical pathology, Volume 71, Issue 4	Increased incidence of BK virus viraemia among patients undergoing chronic haemodialysis: a case–control study	Samer Fuad Swedan
27	ASIAN J TROP BIOTECHNOL ISSN: 0216-6887 Volume 18, Number 1, June 2021 E-ISSN: 2301-8658 Pages: 13-27	Analysis of purity and concentration of DNA extracted from intron patho gene-spin extraction on crab processed food product samples	ALFI SOPHIAN♥, RATNA PURWANINGSIH, MUINDAR, EKA PUTRI JUNIARTI IGIRISA, MUHAMMAD LUTHFI AMIRULLAH
28	APS Publications	First Report of Fig Mosaic Virus on Fig in Russia	S. Chirkov, S. Tsygankova, S. Rastorguev, I. Mitrofanova, S. Chelombit, E. Boulygina, N. Slobodova, and F. Sharko
29	APS Publications	First Report of Duranta leaf curl virus Infecting Ficus virens Showing Leaf Curl Symptoms in Pakistan	Aamir Lal, Eui-Joon Kil, Thuy Thi Bich Vo, Chairina Fadhila, Phuong Thi Ho, Malik Nawaz Shuja, Muhammad Ali, and Sukchan Lee
30	Wiley Online Library	Molecular characterization of infectious bronchitis virus based on RNA-dependent RNA polymerase gene	Mozafar Hajijafari Anaraki,Nariman Sheikhi,Hadi Haghbin Nazarpak,Gholamreza Nikbakht Brujeni
31	Iran J Vet Res. 2021 Spring; 22(2): 155–160.	Molecular characterization and phylogenetic analysis of VIIl sub-genotype of avian orthoavulavirus 1	M. Khosravi,1,* S. Seifi,2 and Z. Tazeh3
32	Al-Qadisiyah journal of pure science	The effect of age ,gender, type of feeding and receiving atreatment on the distribution of Enterovirus infection among chidren	Mohammed Sarim Al-fatlawi, Ghaidaa J. Mohammed
33	Research, 10 Jan 2023	An Effective Integrated Machine Learning Framework for Identifying Severity of Tomato Yellow Leaf Curl Virus and Their Experimental Validation	NATTANONG BUPI, VINOTH KUMAR SANGARAJU, LE THI PHAN, AAMIR LAL, THUY THI BICH VO, PHUONG THI HO, MUHAMMAD AMIR QURESHI, MARJIA TABASSUM, SUKCHAN LEE , AND BALACHANDRAN MANAVALAN

TroubleShooting Guide

QAfter infecting virus gene with cell line, experiment for checking cell condition has performed. Is it possible to only extract virus gene in order to use it for sequencing.: AThis kit not only extract virus but also genome(DNA/RNA). Therefore, it is recommended to sequester the target virus gene by amplifying and purifying the target virus gene by PCR.

QI am willing to extract only virus DNA, can lysis buffer lysed host’s nucleus membrane?: AThe nucleus membrane can also be lysed with the lysis buffer in the kit. Therefore, not only the virus gene but also the genome (gDNA, RNA) of the host cell can be extracted. This extracted DNA can be used as a sample for the detection of virus using PCR but it is impossible to isolate the nucleic acid of pure virus. The confirmation of virus infection can be determined by PCR or RT-PCR.

QI want to confirm infection of PCV from Pig’s tissue. Is it possible to extract viral DNA from tissue? If this works, please provide me protocol.: AThis kit is possible to extract viral gene from tissue. Generally, we need to prepare 10 to 25mg of sample, but we recommend 50mg for heart, brain muscle tissues. The protocol recommends to homogenize the Tissue and add PBS to make a total volume of 150ul. Then, move onto next step.

QWe are currently developing a type B infection treatment. We are planning to extract HBV from HBV infected blood.: AWe recommend Viral-gene spin Viral DNA/RNA Extraction kit. Viral-gene spin product has designed to easily extract viral genes from samples of various liquid components such as serum, plasma and cell culture within 20 minutes.

QWhen we apply the HBV infected blood sample for PCR using designed primer, however, no bands are shown while performing amplification. What is the solution?: AThis product is a product that can extract DNA or RNA virus from various liquids such as serum, plasma, etc., various liquid such as tissue emulsion liquid, feces suspension liquid, and cell culture liquid. You should first check the purity and yield of the extracted HBV sample and design the appropriate PCR condition accordingly. I think it is better to run PCR after confirming the specificity and sensitivity of the designed primer.