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Life Science (Reagent) Extraction Reagent DNA

i-genomic Plant DNA Extraction Mini Kit

Cat.No	Capacity	Inquire
17371	50 col.

Inquire List

Description
TroubleShooting Guide
Related products
Protocol
MSDS

PRODUCT INFORMATION

Description

Spin type product for extracting purified DNA from plant specimen as well as processed food

• Specialized in extracting DNA from various plant samples such as Gramineae and seed as well as processed food
• High reliability and reproducibility by providing optimized 5 types of protocol for each sample
• Possible to extract high yield and purified DNA from plant containing secondary product by using Enhancer Solution
• Standard sample description and data are provided for more than 100 types of plant specimen and processed food
• Concrete instruction is provided to extract DNA easily

The i-genomic™ Plant DNA Extraction Mini Kit extracts genomic DNA more easily and quickly from processed foods such as wheat, high pressure, hot water, juice, and sterilization as well as plant samples such as leaves, stem, roots, fruits and seeds Especially, Gramineae, which has a weak secondary coat compared to other seeds, can be used for extracting plant DNA. It is possible to use PPT Buffer and Enhancer Solution to prevent from interfering with secondary product. It is designed and developed to extract high purified genomic DNA. In addition, since GMO labeling of processed foods using GMO agricultural products is mandatory, this product can extract genomic DNA from plants as well as processed foods. A protocol to extract Genomic DNA from various plant samples was tested using over 100 plant samples to obtain genomic DNA extraction results (see Figure 1) And related data (see Technical Data).

Figure 1. Plant Samples Applied by Standard Protocol

i-genomic™ Series DNA Extraction Mini Kit Kit is specialized for extracting genomic DNA from specific specimen. (Figure 2. Reference). Also, specialized tools such as lysis buffer and inhibitor remover are provided for each sample. Therefore, i-genomic™ Series DNA Extraction Mini Kit is reliable for extracting high yield and purified genomic DNA and provides detailed protocol for high reproducibility. i-genomic™ Series DNA Extraction Mini Kit provides CAPS which packed each column in order to use in pathogen research and minimize contamination. (Figure 3. reference). Therefore, i-genomic™ Series DNA Extraction Mini Kit is applied for not only pathogen research but also SNP genotyping, RAPD analysis, AFLP analysis, RFLP analysis, Microsatellite analysis.

Figure 2. Six kinds of i-genomic™ Series DNA Extraction Mini Kits

Figure림 3. Spin Column & CAPS Information

Applications

01PCR & Real-time PCR
02Southern blotting
03SNP
04Gene cloning
05Genotyping
06Pathogen research etc.

Kit Contents

Contents	50 COLUMNS
Buffer PG (Lysis Buffer)	30ml × 1 ea
Buffer PPT (Precipitation Buffer)	7ml × 1 ea
Buffer PB	50ml × 1 ea
Buffer PWA	40ml × 1 ea
Buffer PWB(Concentration)	10ml × 1 ea
Buffer PE	20ml × 1 ea
Enhancer Solution	0.5 ml x 1 tube
Spin Column(Green color O-ring)	50 Columns
Collection Tube	100 tubes
RNase A, lyophilized	3 mg x 1 tube
Proteinase K, lyophilized	22 mg x 1 tube
Manual	1 ea

Technical Data

Observation for yield and purity of genomic DNA from various plant specimen

Citation List

1	Plant Molecular Biology June 2016, Volume 91, Issue 3, pp 355–374	Intron retention and rhythmic diel pattern regulation of carotenoid cleavage dioxygenase 2 during crocetin biosynthesis in saffron	Oussama Ahrazem, Angela Rubio-Moraga, Javier Argandoña-Picazo, Raquel Castillo, Lourdes Gómez-GómezEmail author	Spain
2	Turkish Journal of Botany, 2017	Genetic diversity and molecular characterization of natural Pancratium maritimum L. populations by DNA markers	CEREN ELİBOL, BEHİYE BANU BİLGEN	Turkey
3	SCIENCE CONFERENCE, 2018	COMPARISON OF DIFFERENT DNA MARKERS FOR SELECTION OF HIGH OLEIC TYPE SUNFLOWER GENOTYPES	BB BILGEN, G EVCI, Y KAYA	Turkey
4	Polar Science Available online 8 December 2018	Photosynthetic response and DNA mutation of tropical, temperate and polar Chlorella under short-term UVR stress	JWS Lai, PE Lim, CY Wong, SM Phang, J Beardall	Malaysia
5	Food Control Volume 89, July 2018, Pages 227-234	Evaluation of locked nucleic acid and TaqMan probes for specific detection of cashew nut in processed food by real time PCR	África Sanchiz a, Isabel Ballesteros b, Eric Marqués c, M. Carmen Dieguez d, Julia Rueda c, Carmen Cuadrado a1, Rosario Linacero c, 1	Spain
6	Volume165, Issue2 Special Issue: Stress Combination February 2019	Regulation by biotic stress of tannins biosynthesis in Quercus ilex: crosstalk between defoliation and Phytophthora cinnamomi infection	Alejandro Gallardo, David Morcuende, Alejandro Solla, Gerardo Moreno, Fernando Pulido, Alberto Quesada	Spain

TroubleShooting Guide

QIs it possible to use gDNA prep for GMO test in vegetable beverages?: AIt seems to be applicable if you separate the vegetable beverages separately. The appropriate protocol is considered to be applicable to Type E protocol (fruit). However, if applied directly in solution, the lysis itself may be diluted and not be prepped.

QIf you want to extract gDNA from lyophilized leaf, please recommend suitable product.: AApply Type A protocol to i-genomic Plant. At this time, the lyophilized leaves are in the form of powder and do not require any homogenization, so use a small amount (5mg) of sample with little moisture content.

QThe gDNA prep is in progress in dry stem. Since the amount of supernatant is very small after PPT, is not it a product problem?: AGenerally, in fresh stem tissues, 350 ~ 400ul of supernatant is formed after the PPT step, but dried stem has a low water content and about 150 ~ 200ul supernatant is formed. This is a feature of the sample and can be done according to the protocol.

QI would like to extract DNA from sunflower seeds. I want to apply protocol G, but why is it less than other protocols?: AGramineae Seed is weaker than other seeds and contains a lot of secondary products. Since the outer skin is thin and light, adding lysis buffer will float on top of the buffer. In addition, when the lysis process is carried out, the buffer is faded and blown.

QIs not the protocol's binding capacity presented?: AThe loading capacity, DNA binding capacity and elution volume are indicated on the "Notice Before Use" for each part.